Apostle MiniMax High Efficiency Cell-Free DNA Isolation Kit

Apostle partners with Beckman Coulter to offer the Apostle MiniMaxTM High Efficiency Isolation Kit. To place an order, or for more information, please visit Beckman Coulter's website:

 
https://www.beckman.com/reagents/genomic/dna-isolation/from-plasma

For more information about this partnership: https://www.beckman.com/news/liquid-biopsy-partnership-with-apostle

• Check our data brochure 
• User guidance: USER VALIDATION GUIDE
• Technical support: support@apostlebio.com

The superb performance of Apostle MiniMax High Efficiency Cell-Free DNA Isolation Kit has been extensively validated.  Please review the examples of publications and data points below.

For a full list of publications, please visit the Publications page:

Apostle MiniMaxTM technology offers a best-in-class efficiency and purity compared with conventional technologies to capture and isolate the circulating cell-free genetic materials. It has been trusted by many of the world's most prestigious leaders in life sciences.

"...This includes recognizing that an innovative product from a startup like Apostle is the best technology to address these needs." said Steve Wowk, Director of Genomics, Beckman Coulter Life Sciences.

cfDNA is a group of highly fragmented DNA molecules, with major peak at ~170bp, doublet peak at ~340bp, triplet peak at ~510bp, and so on. Therefore, cfDNA isolation kit capable of highly efficient cfDNA isolation spanning wide cfDNA size distribution is desired. Apostle MiniMax High Efficiency cfDNA Isolation Kit meets such need as demonstrated from its >95% recovery of DNA ladder. This is further validated through isolation of natural cfDNA from human plasma (Exhibit 1A) and urine samples (Exhibit 1B), where Apostle MiniMax High Efficiency cfDNA Isolation Kit offers superior cfDNA isolation efficiency over wide range, specifically covering the 170bp, 340bp, and 510bp cfDNA peaks, when compared with major alternative product.  Apostle MiniMax High Efficiency Cell-Free DNA Isolation Kit enables superior and consistent performance of DNA mutation detection, validated by qPCR (Exhibit 2).

A) Cell-free plasma was separated from blood samples by centrifugation for 10 minutes at 2000g at 4oC, then centrifuged for 10 minutes at 16000g at 4oC. cfDNA was isolated from 4mL plasma with Apostle MiniMax High Efficiency cfDNA Isolation Kit (red curve) and major alternative product (blue curve). The isolated cfDNA was characterized by Bioanalyzer 2100. 

B) Cell-free urine was prepared by centrifugation for 10 minutes at 16000g at 4oC. cfDNA was isolated from 20mL urine with Apostle MiniMax High Efficiency cfDNA Isolation Kit (red curve) and major alternative product (blue curve). The isolated cfDNA was characterized by Bioanalyzer 2100. Apostle MiniMaxTM High Efficiency cfDNA Isolation Kit offers superior cfDNA isolation efficiency for both plasma and urine samples.

20 uL of DNA fragment containing the EGFR c.2573T>G L858R mutation (synthetic, ~170 bp), with concentration of 1 ng/uL, 0.1 ng/uL, 0.01 ng/uL, 0.001 ng/uL, was spiked into 1mL TE buffer (blue) or Serum (red) respectively. The mutated DNA fragment was isolated with Apostle MiniMax High Efficiency Cell-Free DNA Isolation Kit (Standard Edition), with a final elution volume of 20 uL. qPCR was performed using 1 uL of the isolated DNA, and compared with 1 uL of the corresponding original mutated DNA solution at 1 ng/uL, 0.1 ng/uL, 0.01 ng/uL, 0.001 ng/uL. 

A) Amplification plot showing highly overlapping curves for mutated DNA fragment isolated with Apostle MiniMaxTM High Efficiency Cell-Free DNA Isolation Kit and original DNA solution at different concentrations. 

B) qPCR standard curve generated using original mutated DNA solution, in order to quantify the recovery of DNA isolated with Apostle MiniMax High Efficiency Cell-Free DNA Isolation Kit. DNA isolation recovery rate was calculated to be >90%. 

Note: Displayed DNA concentration series at 1, 0.1, 0.01, 0.001 ng/ul are the concentrations of the original DNA dilution series before spiking into 1mL of serum. The corresponding DNA isolation working concentrations are 20 pg/ul, 2 pg/ul, 0.2 pg/ul, 0.02 pg/ul, respectively.