Product Introduction
RiboCraft Alpha Transfection Reagent is a one-of-a-kind transfection reagent, uniquely suited for both in vitro and in vivo RNA transfection. This dual-function capability is a rare find in the market, offering unparalleled convenience and efficiency for researchers exploring the frontiers of genetic manipulation. RiboCraft Alpha provides any research program the unique ability to directly translate from cell-based assays to animal studies.
RiboCraft Alpha Transfection Reagent (in vitro) is a cutting-edge transfection reagent engineered to deliver RNA cargos, including mRNA, siRNA, and gene-editing tools, across a variety of cell lines. Its exceptional robustness and versatility ensure consistent and highly efficient transfection under different experimental conditions, supporting a broad range of applications from basic research to preclinical studies.
Product overview
Key Features
| Wide Compatibility |
Compatible with a wide variety of cell types and tissues. |
| Efficient Multi-functional Transfection |
Capable of efficiently delivering different types of RNA cargos, including gene editing tools. |
| Easy-to-Use Method |
Only requires a one-step mixing process for use, making it easy to operate with high encapsulation rates and good reproducibility. |
| Flexible Dose Range |
Supports RNA delivery across a wide range of doses, meeting diverse application scenarios. |
| Robustness and Reproducible Formulation |
Maintains consistent and reproducible transfections across various incubation times and temperatures. |
| Buffer and Serum Tolerance |
Compatible with various buffers, pH environments, culture media, and demonstrates good tolerance to serum, making it adaptable to different experimental needs. |
| Extrahepatic Targeting |
Efficient and consistent RNA delivery in animal models, primarily exhibiting spleen tropism. |
| Storage Conditions |
Store at 2 – 8 °C; can be frozen at -20 °C for extended storage. |
| Shelf Life |
Valid for 1 year. |
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Performance
RiboCraft Alpha is an RNA delivery tool that demonstrates outstanding RNA gene expression and transfection efficiency across a wide range of cell lines. It possesses stable physicochemical properties; it is colorless, odorless, water-soluble, and highly safe. Its simple usage can be easily implemented under standard laboratory conditions, and once prepared, it remains stable at room temperature for up to 30 minutes, ensuring the reproducibility and consistency of results.
Exhibit 1: RiboCraft Alpha Transfection Reagent (in vitro) has demonstrated extremely high levels of gene expression and transfection efficiency across multiple cell lines. Fluorescence microscopy images of tested cell lines were captured 24 hours post-transfection to visualize eGFP expression levels.
Exhibit 2: RiboCraft Alpha Transfection Reagent (in vitro) demonstrated high transfection efficiency in vitro.
Moreover, experimental data show that RiboCraft Alpha not only maintains compatibility with different types of physiological buffers but also exhibits stable transfection efficiency under pH conditions ranging from 4 to 8. It demonstrates serum tolerance during mRNA transfection and exhibits excellent stability across various temperatures. This key feature enhances its practicality in complex biological environments. Additionally, by adjusting the ratio of RiboCraft Alpha to mRNA, it can achieve high transfection efficiency across a wide range of mRNA doses, expanding its applicability in various delivery scenarios.
Table 1: The recommended reagent amounts for mRNA transfection assays in 96-well plates are listed below.
Table 2: mRNA transfection scaling up and down table. Recommended dosage for assays of different scales are list below.
Note: The amounts listed in this table are for reference only. The specific usage of mRNA and transfection reagents should be optimized based on the cell type and other experimental conditions.
Table 3: For siRNA transfection experiments in a 48-well plate, the recommended reagent quantities are as follows:
Table 4: siRNA transfection scaling up and down table. Recommended dosage for assays of different scales are list below.
Note: The amounts listed in this table are for reference only. The specific usage of mRNA and transfection reagents should be optimized based on the cell type and other experimental conditions.
In summary, not only does RiboCraft Alpha outperform industry-standard reagents in mRNA transfection, but it also shows superior results in siRNA tests. Its powerful performance extends to gene editing, successfully delivering Cas9 mRNA and sgRNA, achieving up to 95% editing efficiency in vitro. RiboCraft Alpha is positioned as a versatile and powerful tool, poised to accelerate research and development in the fields of RNA therapeutics and technologies.
