MiniGenomics ® Multi-Sample DNA Isolation Kit is designed for rapid extraction and purification of high-quality, ready-to-use genomic DNA from multiple sample types, including but not limited to saliva, buccal swab, genital tract swab, tissue/cultured cell, and peripheral blood buffy coat.
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Sample type |
Multiple sample types, including but not limited to - tissue / cultured cell - saliva - buccal swab - peripheral blood buffy coat |
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Sample amount |
1-200 µl
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Cat #
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A180911-200 |
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Capacity
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200 preps |
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Manual protocol
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Protocol for Peripheral blood buffy coat |
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Time per run (manual)
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50-70 minutes
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MSDS
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Download
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MagTouch 2000 automation programs & instructions
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Program Installation instruction:
1. Download "Items.zip" file and unzip, save the folder to the root directory of a flash drive.
2. Plug the flash drive into the USB port at the back of MagTouch 2000.
3. Press Settings > Im.&export > Import. Select the program(s) by pressing the checkmark once (check mark symbol will turn
green
) and press import.
Any questions? Please send an email to
support@apostlebio.com or call support hotline at 888-305-3218 (Mon-Fri: 9AM to 6PM, PST)
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Features
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Novel and original magnetic nanoparticle technology for DNA adsorption and releasing
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Standard purification methods for biological samples stored in multiple forms or reagents
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Special reagent for effectively enhancing the quality of DNA of challenging samples
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Optimized standardized protocol for multiple kinds of biological samples to one kit
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Compatible with multiple high-throughput automated nucleic acid extraction platforms
Performance of Isolating Genomic DNA from Cell Lines
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| Exhibit 1: DNA extraction of blood samples from 2 kinds of cell lines (Human MSCs, 1*10 6 cells; Human 293 cells; 8*10 6 cells) by MiniGenomics® Multi-Sample DNA Isolation Kit. DNA product (1%) was detected by agarose gel electrophoresis. |
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| Exhibit 2: qPCR detection of human β-globin gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis. Ct values are shown in the table below. Absolute value of mean Ct (MSCs) - mean Ct(293 cells) is 3.069 (293 cells number : MSCs number=8:1). |
| Table 1: Qualitative and quantitative analysis of gDNA extracted from the cell culture samples on Nanodrop 2000. |
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Performance of Isolating Genomic DNA from Saliva
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| Exhibit 3: DNA extraction of saliva samples from 3 volunteers (sample ids: LJ, ZLH, ZF) by MiniGenomics® Multi-Sample DNA Isolation Kit. DNA product (1%) was detected by agarose gel electrophoresis. |
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| Exhibit 4: qPCR detection of human β-globin gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis. Ct values are shown in the table below. |
| Table 2: Qualitative and quantitative analysis of gDNA extracted from the saliva samples on Nanodrop 2000. |
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