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Apostle MiniMax DNA Universal Library Preparation Kit


Introduction

Apostle MiniMax DNA Universal Library Preparation Kit (for Illumina) is designed for the preparation of high-quality libraries from double-stranded DNA (dsDNA) on Illumina platforms. This A-T ligation-based kit offers a stable and efficient library preparation solution for a variety of applications.

Features

o   Suitable for various sample types including FFPE DNA

o   High complexity libraries generated from a wide range of input amounts.

o   Adapters with unique dual indexes (UDIs) to reduce impact of index hopping on Illumina® platforms.

o   Compatible with unique molecular identifiers (UMI) adapters to enable confident calling of low-frequency variants.

o   Purification beads and optimized size selection methods included.

Applications

Application

 Sample Type *

 Input recommended

Whole Genome Sequencing

Animal or plant gDNA (without serious degradation)
Cell-free DNA
Microbial DNA (without serious degradation)

50-500 ng

≥ 1ng (analytical sensitivity dependent)

1-500 ng

Targeted sequencing

gDNA (without serious degradation)
FFPE DNA (with average size > 500 bp)
Cell-free DNA

 ≥ 50 ng (quality dependent) **
≥ 100 ng (quality dependent) **
≥ 1 ng (analytical sensitivity dependent)

* This kit is suitable for fragmented gDNA/FFPE DNA or cfDNA that does not require fragmentation.

** For gDNA, input recommended here indicates the amount quantified after DNA fragmentation.

Workflow

Performance

  • High Efficiency

  • Unbiased Coverage




  • Accurate Variant Analysis