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MiniGenomics® whole blood DNA Isolation kit

     

Introduction

MiniGenomics® whole blood DNA Isolation Kit is designed for rapid extraction and purification of high-quality, ready-to-use genomic DNA from fresh or frozen whole blood sample containing Citrate, EDTA, or Heparin anticoagulants.

Features

o Advanced magnetic nanoparticle technology for superior DNA adsorption and release

o Standard purification methods suitable for biological samples in various forms or reagents

o Specialized reagents to enhance DNA quality from challenging samples

o Optimized, standardized protocol compatible with diverse biological samples using a single kit

o Compatible with multiple high-throughput automated nucleic acid extraction platforms

Performance

DNA Extraction
Exhibit 1: DNA extraction of blood samples from a volunteer by MiniGenomics® whole blood fast kit and 3 competitor kits (Q, O, and M brands). DNA product (150ng) was detected by agarose gel electrophoresis.
Table 1: Qualitative and quantitative analysis of gDNA extracted from the blood samples on Nanodrop 2000.
DNA Extraction
DNA Extraction
Exhibit 2: qPCR detection of human β-globin gene level in extracted DNA of all samples. 1% of extracted DNA product were loaded in qPCR assay, and 1/10 of PCR product were analyzed by agarose gel electrophoresis. Ct values are shown in the table below.

Products

Sample type

Fresh or Frozen whole blood samples (may contain Citrate, EDTA, or Heparin anticoagulants)

Sample amount

1-200 µl

Cat #

A180903-50 (50 preps)

A180903-200 (200 preps)

Capacity

50 preps or 200 preps

Manual protocol

Protocol download  (A180903-50 for 50 preps)

Protocol download  (A180903-200 for 200 preps)

Time per run (manual)

50-60 minutes

MSDS

Download

MagTouch 2000 automation programs & instructions

Automation protocol download  

 

MagTouch2000 program download

 

Program Installation instruction:

 

1.     Download "Items.zip" file and unzip, save the folder to the root directory of a flash drive.

2.     Plug the flash drive into the USB port at the back of MagTouch 2000.

3.   Press  Settings >  Im.&export >  Import. Select the program(s) by pressing the checkmark once (check mark symbol will turn green) and press import.

 

Any questions? Please send an email to support@apostlebio.com  or call support hotline at 888-305-3218 (Mon-Fri: 9AM to 6PM, PST)

A new clinical study, led by scientists from MD Anderson Cancer Center and published in Cancer Cell (journal impact factor = 50.3), shows that tumor and cfDNA methylation can be used to identify SCLC subtypes and might guide precision SCLC therapy. Apostle MiniMax cfDNA kit is one of the critical commercial assays listed in this article. Congratulations to this clinical research team. To date, the Apostle MiniMax technology has been used in 2 articles published in Nature Communications, 2 in Nature Medicine, 1 in Science Translational Medicine, 1 in PNAS, and over 60 scientific articles by over 60 international research and clinical teams in different journals.