Introduction
MiniGenomics® whole blood DNA Isolation Kit is designed for rapid extraction and purification of high-quality, ready-to-use genomic DNA from fresh or frozen whole blood sample containing Citrate, EDTA, or Heparin anticoagulants.
Features
o Advanced magnetic nanoparticle technology for superior DNA adsorption and release
o Standard purification methods suitable for biological samples in various forms or reagents
o Specialized reagents to enhance DNA quality from challenging samples
o Optimized, standardized protocol compatible with diverse biological samples using a single kit
o Compatible with multiple high-throughput automated nucleic acid extraction platforms
Performance
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Exhibit 1: DNA extraction of blood samples from a volunteer by MiniGenomics® whole blood fast kit and 3 competitor kits (Q, O, and M brands). DNA product (150ng) was detected by agarose gel electrophoresis. |
Table 1: Qualitative and quantitative analysis of gDNA extracted from the blood samples on Nanodrop 2000. |
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Exhibit 2: qPCR detection of human β-globin gene level in extracted DNA of all samples. 1% of extracted DNA product were loaded in qPCR assay, and 1/10 of PCR product were analyzed by agarose gel electrophoresis. Ct values are shown in the table below. |
Products
Sample type
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Fresh or Frozen whole blood samples (may contain Citrate, EDTA, or Heparin anticoagulants)
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Sample amount
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1-200 µl
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Cat #
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A180903-50 (50 preps)
A180903-200 (200 preps)
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Capacity
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50 preps or 200 preps
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Manual protocol
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Protocol download
(A180903-50 for 50 preps)
Protocol download
(A180903-200 for 200 preps)
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Time per run (manual)
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50-60 minutes
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MSDS
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Download
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MagTouch 2000 automation programs & instructions
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Program Installation instruction:
1. Download "Items.zip" file and unzip, save the folder to the root directory of a flash drive.
2. Plug the flash drive into the USB port at the back of MagTouch 2000.
3. Press Settings > Im.&export > Import. Select the program(s) by pressing the checkmark once (check mark symbol will turn green) and press import.
Any questions? Please send an email to support@apostlebio.com
or call support hotline at 888-305-3218 (Mon-Fri: 9AM to 6PM, PST)
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