Performance with fresh or frozen tissue, cultured cells
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| Exhibit 1: DNA extraction of blood samples from 2 kinds of cell lines (Human MSCs, 1*106 cells; Human 293 cells; 8*106 cells) by MiniGenomics® Multi-Sample DNA Isolation Kit. DNA product (1%) was detected by agarose gel electrophoresis. |
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| Exhibit 2: qPCR detection of human β-globin gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis. Ct values are shown in the table below. Absolute value of mean Ct (MSCs) - mean Ct(293 cells) is 3.069 (293 cells number : MSCs number=8:1). |
| Table 1: Qualitative and quantitative analysis of gDNA extracted from the cell culture samples on Nanodrop 2000. |
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Performance with Saliva Samples
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| Exhibit 3: DNA extraction of saliva samples from 3 volunteers (sample ids: LJ, ZLH, ZF) by MiniGenomics® Multi-Sample DNA Isolation Kit. DNA product (1%) was detected by agarose gel electrophoresis. |
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| Exhibit 4: qPCR detection of human β-globin gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis. Ct values are shown in the table below. |
| Table 2: Qualitative and quantitative analysis of gDNA extracted from the saliva samples on Nanodrop 2000. |
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Performance with genital tract swab samples
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| Exhibit 5: DNA extraction of genital tract swab samples from 6 volunteers by MiniGenomics® Multi-Sample DNA Isolation Kit. DNA product (1/20) was detected by agarose gel electrophoresis. |
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| Exhibit 6: qPCR amplification plots of human β-globin gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis. |
| Table 3: Qualitative and quantitative analysis of gDNA extracted from the genital tract swab samples on Nanodrop 2000. |
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