MiniGenomics ® whole blood DNA Isolation Kit is designed for rapid extraction and purification of high-quality, ready-to-use genomic DNA from fresh or frozen whole blood sample containing Citrate, EDTA, or Heparin anticoagulants.
Sample type | Fresh or Frozen whole blood samples (may contain Citrate, EDTA, or Heparin anticoagulants) |
Sample amount | 1-200 µl |
Cat # | A180903-50 |
Capacity | 50 preps |
Manual protocol | |
Time per run (manual) | 50-60 minutes |
MSDS | Download |
MagTouch 2000 automation programs & instructions |
Program Install instruction: 1. Download "Items.zip" file and unzip, save the folder to the root directory of a flash drive. 2. Plug the flash drive into the USB port at the back of MagTouch 2000. 3. Press Settings > Im.&export > Import. Select the program(s) by pressing the checkmark once (check mark symbol will turn green ) and press import. Any questions? Please send an email to support@apostlebio.com or call support hotline at 888-305-3218 (Mon-Fri: 9AM to 6PM, PST) |
Features
o Advanced magnetic nanoparticle technology for superior DNA adsorption and release
o Standard purification methods suitable for biological samples in various forms or reagents
o Specialized reagents to enhance DNA quality from challenging samples
o Compatible with multiple high-throughput automated nucleic acid extraction platforms
Performance
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| Exhibit 1: DNA extraction of blood samples from a volunteer by MiniGenomics® whole blood fast kit and 3 competitor kits (Q, O, and M brands). DNA product (150ng) was detected by agarose gel electrophoresis. |
| Table 1: Qualitative and quantitative analysis of gDNA extracted from the blood samples on Nanodrop 2000. |
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| Exhibit 2: qPCR detection of human β-globin gene level in extracted DNA of all samples. 1% of extracted DNA product were loaded in qPCR assay, and 1/10 of PCR product were analyzed by agarose gel electrophoresis. Ct values are shown in the table below. |
