MiniGenomics® Stool DNA Isolation Kit is designed for rapid extraction and purification of high-quality, ready-to-use genomic DNA and/or bacterial DNA from fresh or frozen stool samples.
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Sample type |
Fresh or frozen stool sample |
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Cat # |
A181206-200 |
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Capacity |
200 preps |
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Manual protocol |
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Time per run (manual) |
In total 50~70 minutes for manual operation, including - 20~40 minutes pre-treatment and lysis time depending on sample size and status - 10 minutes binding time - 15 minutes washing time - 5 minutes elution time |
Features
o Advanced magnetic nanoparticle technology for superior DNA adsorption and release
o Standard purification methods suitable for biological samples in various forms or reagents
o Specialized reagents to enhance DNA quality from challenging samples
o Compatible with multiple high-throughput automated nucleic acid extraction platforms
Performance of Isolating Genomic DNA from Stool Samples
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| Exhibit 1: DNA extraction of stool samples from 4 volunteers by MiniGenomics® Stool DNA Isolation Kit and a competitor kit (Q brand). DNA product (1/20) was detected by agarose gel electrophoresis. |
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| Exhibit 2: qPCR detection of human β-globin gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis. Ct values are shown in the table below. |
| Table 1: Qualitative and quantitative analysis of gDNA extracted from the stool samples on Nanodrop 2000. |
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Performance of Isolating Bacterial DNA from Stool Samples
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| Exhibit 3: DNA extraction of stool samples from 2 volunteers by MiniGenomics® Stool DNA Isolation Kit. DNA product (1/20) was detected by agarose gel electrophoresis. |
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| Exhibit 4: qPCR detection of bacterial 16SrRNA gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis. |
