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Apostle MiniMax DNA Universal Library Preparation Kit


Introduction

Apostle MiniMax DNA Universal Library Preparation Kit (for Illumina) is designed for the preparation of high-quality libraries from double-stranded DNA (dsDNA) on Illumina platforms. This A-T ligation-based kit offers a stable and efficient library preparation solution for a variety of applications.

Features

o   Suitable for various sample types including FFPE DNA

o   High complexity libraries generated from a wide range of input amounts.

o   Adapters with unique dual indexes (UDIs) to reduce impact of index hopping on Illumina® platforms.

o   Compatible with unique molecular identifiers (UMI) adapters to enable confident calling of low-frequency variants.

o   Purification beads and optimized size selection methods included.

Applications

Application

 Sample Type *

 Input recommended

Whole Genome Sequencing

Animal or plant gDNA (without serious degradation)
Cell-free DNA
Microbial DNA (without serious degradation)

50-500 ng

≥ 1ng (analytical sensitivity dependent)

1-500 ng

Targeted sequencing

gDNA (without serious degradation)
FFPE DNA (with average size > 500 bp)
Cell-free DNA

 ≥ 50 ng (quality dependent) **
≥ 100 ng (quality dependent) **
≥ 1 ng (analytical sensitivity dependent)

* This kit is suitable for fragmented gDNA/FFPE DNA or cfDNA that does not require fragmentation.

** For gDNA, input recommended here indicates the amount quantified after DNA fragmentation.

Workflow

Performance

  • High Efficiency

  • Unbiased Coverage




  • Accurate Variant Analysis
A new clinical study, led by scientists from MD Anderson Cancer Center and published in Cancer Cell (journal impact factor = 50.3), shows that tumor and cfDNA methylation can be used to identify SCLC subtypes and might guide precision SCLC therapy. Apostle MiniMax cfDNA kit is one of the critical commercial assays listed in this article. Congratulations to this clinical research team. To date, the Apostle MiniMax technology has been used in 2 articles published in Nature Communications, 2 in Nature Medicine, 1 in Science Translational Medicine, 1 in PNAS, and over 60 scientific articles by over 60 international research and clinical teams in different journals.