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MiniGenomics® FFPE Tissue DNA Isolation kit

  

Introduction

MiniGenomics® FFPE Tissue DNA Isolation Kit is designed for rapid extraction and purification of high-quality, ready-to-use genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue.

Features

o Advanced magnetic nanoparticle technology for superior DNA adsorption and release

o Standard purification methods suitable for biological samples in various forms or reagents

o Specialized reagents to enhance DNA quality from challenging samples

o Compatible with multiple high-throughput automated nucleic acid extraction platforms

Performance

DNA Extraction
Exhibit 1:
(A) DNA extraction of rat liver FFPE tissue samples (about 4 micron square, 2 slides/group, preserved more than 2 years) by MiniGenomics® FFPE Tissue DNA Isolation kit and Competitor kit (P brand). DNA product (1/20) was detected by agarose gel electrophoresis.
(B) qPCR detection of rat GAPDH gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis. Ct values are shown in the table below.
Table 1: Qualitative and quantitative analysis of gDNA extracted from the FFPE samples on Nanodrop 2000.
DNA Extraction

Products

Sample type

Formalin-Fixed, Paraffin-Embedded (FFPE) tissue

Cat #

A180905-50 (50 preps)

A180905-200 (200 preps)

Capacity

50 preps or 200 preps

Manual protocol

Protocol download  (A180905-50 for 50 preps)

Protocol download  (A180905-200 for 200 preps)

Time per run (manual)

 -  2.5~17.5 hrs for manually pre-treatment and lysis time depending on sample size and status
 -  10 minutes binding time
 -  15 minutes washing time
 -  5 minutes elution time

MSDS

Download

MagTouch 2000 automation programs & instructions

Automation protocol download  

 

MagTouch2000 program download

 

Program Installation instruction:

 

1.     Download "Items.zip" file and unzip, save the folder to the root directory of a flash drive.

2.     Plug the flash drive into the USB port at the back of MagTouch 2000.

3.   Press  Settings >  Im.&export >  Import. Select the program(s) by pressing the checkmark once (check mark symbol will turn green) and press import.

 

Any questions? Please send an email to support@apostlebio.com  or call support hotline at 888-305-3218 (Mon-Fri: 9AM to 6PM, PST)