Menu
Your Cart

MiniGenomics® Stool DNA Isolation Kit

   

Introduction

MiniGenomics® Stool DNA Isolation Kit is designed for rapid extraction and purification of high-quality, ready-to-use genomic DNA from fresh or frozen stool samples.

Features

o Advanced magnetic nanoparticle technology for superior DNA adsorption and release

o Standard purification methods suitable for biological samples in various forms or reagents

o Specialized reagents to enhance DNA quality from challenging samples

o Compatible with multiple high-throughput automated nucleic acid extraction platforms

Performance of Isolating DNA from Stool Samples

DNA Extraction
Exhibit 1: DNA extraction of stool samples from 4 volunteers by MiniGenomics® Stool DNA Isolation Kit and a competitor kit (Q brand). DNA product (1/20) was detected by agarose gel electrophoresis.
DNA Extraction
Exhibit 2: qPCR detection of human β-globin gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis. Ct values are shown in the table below.
Table 1: Qualitative and quantitative analysis of gDNA extracted from the stool samples on Nanodrop 2000.
DNA Extraction

Performance of Isolating Pathogen DNA from Stool Samples

DNA Extraction
Exhibit 3: DNA extraction of stool samples from 2 volunteers by MiniGenomics® Stool DNA Isolation Kit. DNA product (1/20) was detected by agarose gel electrophoresis.
DNA Extraction
Exhibit 4: qPCR detection of bacterial 16SrRNA gene level in extracted DNA of all samples. PCR product (1%) were analyzed by agarose gel electrophoresis.

Products

Sample type

Fresh or frozen stool sample

Cat #

A181206-50 (50 preps)

A181206-200 (200 preps)

Capacity

50 preps or 200 preps

Manual protocol

Protocol download  (A181206-50 for 50 preps)

Protocol download  (A181206-200 for 200 preps)

Time per run (manual)

In total 50~70 minutes for manual operation, including

 -  20~40 minutes pre-treatment and lysis time depending on sample size and status

 -  10 minutes binding time

 -  15 minutes washing time

 -  5 minutes elution time

MSDS

Download

MagTouch 2000 automation programs & instructions

Automation protocol download  

 

MagTouch2000 program download

 

Program Installation instruction:

 

1.     Download "Items.zip" file and unzip, save the folder to the root directory of a flash drive.

2.     Plug the flash drive into the USB port at the back of MagTouch 2000.

3.   Press  Settings >  Im.&export >  Import. Select the program(s) by pressing the checkmark once (check mark symbol will turn green) and press import.

 

Any questions? Please send an email to support@apostlebio.com  or call support hotline at 888-305-3218 (Mon-Fri: 9AM to 6PM, PST)

A new clinical study, led by scientists from MD Anderson Cancer Center and published in Cancer Cell (journal impact factor = 50.3), shows that tumor and cfDNA methylation can be used to identify SCLC subtypes and might guide precision SCLC therapy. Apostle MiniMax cfDNA kit is one of the critical commercial assays listed in this article. Congratulations to this clinical research team. To date, the Apostle MiniMax technology has been used in 2 articles published in Nature Communications, 2 in Nature Medicine, 1 in Science Translational Medicine, 1 in PNAS, and over 60 scientific articles by over 60 international research and clinical teams in different journals.